Fatih Kocabaş

Moleküler Biyolog ve Genetikçi (PhD Candidate)

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FATIH KOCABAŞ

Graduate School of Biomedical Sciences,
UT Southwestern Medical Center,
Fatih.Kocabas@utsouthwestern.edu


EDUCATION
2007-Present UT Southwestern Medical Center,
Graduate School of Biomedical Sciences,
2002-2006 Middle East Technical University , Turkey
B.S. in Molecular Biology & Genetics with honors
2001-2002 English Preparatory School
1998-2001 Kirikkale High School of Science with honors

PROJECT WORK

2007 Fall-1st Rotation UT Southwestern Medical Center- Johnson Lab, “Identification of Mash1 lineage in the dorsal spinal cord”

***Confocal microscopy, primer design, mouse handling, perfusion assay, cryosectioning, immunohistochemistry


2007 Spring-Summer University of California, San Francisco- Vale Lab, "Chlamydomonas flagellar surface motility and Intraflagellar transport by FIONA ", as a junior specialist

***TIRF microscopy techniques, single molecule imaging, fluorescence imaging with one-nanometer accuracy (FIONA), Chlamydomonas culture, antibody coating of microspheres, winview32 software, Matlab for histogram analysis


2006 Summer-Fall University of California, San Francisco- Fisher Lab, “Epigenetic mechanisms regulating stem cell differentiation in placenta; HIF related histone modifications” as a junior specialist

***Animal Cell Culture Techniques, passages, freezing and thawing of cells, viable cell counting

***Stem cell and epigenetics: Growth of trophoplast stem cells and placental fibroblasts, , RNA isolation from stem cells, western blot, histone extraction, nuclear and cytoplasmic protein extraction from animal cells, differentiation of ESCs and TSCs in vitro, hypoxia chamber usage and hypoxia treatment of cells, ICSI (Intracytoplasmic sperm injection)


2006 Spring Middle East Technical University-Severcan lab, “The Effect of Sleep Deprivation on Rat Spleen Tissue” as a special project student
2005 Summer California Institute of Technology-Stathopoulos Lab, “Identification of transcription factors that recognize A box in ind gene affecting dorsoletaral patterning in Drosophila” as a SURF researcher

***One-Hybrid screens with yeast, yeast culture, competent cells, transformations, plasmid isolations, gene integration, B-Galactosidase assay, collection and fixation of Drosophila embryo, generation of a cDNA


2005 Winter Middle East Technical University-Akkaya Lab, “Determination of genes induced upon stripe rust resistance” as an undergrad researcher
***Classical molecular biology techniques, cloning, plasmid and genomic DNA isolation, agarose gel electrophoresis, SDS PAGE , transformation with plasmid DNA, PCR, RT-PCR, total RNA isolation from animal & plant cells, restriction enzyme digestion & southern transfer, thin layer chromatography, column chromatography, electroporation


AWARDS and HONORS
Summer 2005 Caltech- Summer Undergraduate Research Fellowship
1994-2001 Turkey-Government Scholarship

POSTER&PRESENTATION
August 2007 Ahmet Yildiz, Fatih Kocabas, Wallace D. Marshall, Ronald D. Vale, “The Role of Kinesin and Dynein on Membrane Protein Transport in Intraflagellar Transport”, Department of Cellular and Molecular Pharmacology, University of California, San Francisco
September 2006 A. Skvortsov, F. Kocabas, O. Bozkurt, C. Ozesmi, F. Severcan, “The Effect of Sleep Deprivation on Rat Spleen Tissue: An FTIR Approach”, The XXVIIIth European Congress on Molecular Spectroscopy (EUCMOS XXVIII), Istanbul University
August 2005 SURF Seminar Day, Caltech, “Identification of transcription factors that recognize the ATTCATTCATGA site affecting dorsolateral patterning in Drosophila”

RESEARCH INTERESTS
Developmental Biology, Human Embryology, Stem cells, Regenerative medicine, and Epigenetics

COMPUTER SKILLS & COMPUTING KNOWLEDGE
Course Attended Introduction to C Programming
Software Minitab (Biostatistics Software), PHYLIP programs, and Matlab
Internet Tools Web designing & publishing

LANGUAGES
TURKISH (Native speaker) & ENGLISH (Advanced level)

LEISURE ACTIVITIES
Voluntary works for society, writing, and playing football, traveling,

REFERENCES
Available upon request from METU, Caltech, and UCSF